A Simplified Pyruvic Acid Analysis Suitable for Onion Breeding Programs
نویسندگان
چکیده
منابع مشابه
Development of an automated system for pyruvic acid analysis in onion breeding
An automated system for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding program. The system includes two high performance liquid chromatography (HPLC) pumps, an autosampler, a column heater, a spectrophotometric detector, and an integrator. With this new method, human error can be eliminated with great repeatability (CV 0.9%), and we can ru...
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Modifications to the commonly used Schwimmer and Weston procedure for determining pyruvic acid in onions are proposed. These modifications involve changes in the concentrations and volumes of the reagents and the use of 515 rather than 420 nm as the wavelength for measurements. These changes improve both the linearity and the sensitivity of the assay and eliminate interference from coloured mat...
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Myostatin or growth and differentiation factor 8 (GDF8), has been known as the factor causing double muscling phenotypes in which a series of mutations make the myostatin protein inactive, hence disabling it to regulate the deposition of muscle fibre. This phenotype happens with high frequency in a breed of sheep known as the Texel. Quantitative trait loci (QTL) studies show that a portion of t...
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Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tubercu...
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Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relatively innocuous reagents. The protocol employs a single purification ste...
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ژورنال
عنوان ژورنال: HortScience
سال: 1995
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.30.6.1306